Description
CellAmp Direct RNA Prep Kit RT-PCR (qPCR) is for preparation of templates used in one-step and two-step real-time RT-PCR utilizing a simple method of extraction directly from cultured cells in 96 well-plates or others. It is possible to prepare templates for real-time PCR from cultured cells in approximately 10 minutes. Use of this kit with a one-step real-time RT-PCR kit (i.e. One Step TB GreenEx TaqqRT-PCR Kit) gene expression analysis can be performed in approximately 2 hours. Templates can be prepared directly from a small quantity of cells using this kit, facilitating gene expression analysis by sensitive real-time RT-PCR. Excellent for detection of rare transcripts.