Description
The CellAmp Direct RNA Prep Kit for qRT-PCR and Protein Analysis allows simple, direct extraction directly from cultured cells to prepare templates used for one-step and two-step qRT-PCR. The kit can be used for cells cultured in 96-well plates and is compatible with high-throughput studies. Preparation of templates for real-time PCR from cultured cells takes approximately 10 minutes and complete gene expression analysis (via One Step or Two Step qRT-PCR) is performed in approximately 2 hours. When used in combination with a reverse transcription kit, cDNA templates for real-time PCR can be synthesized in about 30 minutes, providing efficient high-throughput analysis. Templates can be prepared directly from small quantities of cells for analysis by high sensitivity qRT-PCR. Genomic DNA can be easily and efficiently removed using this kit. Excellent for detection of rare transcripts.
