Takara, 6046, BcaBEST™ Labeling Kit

The BcaBEST Labeling Kit is a novel random primer labeling system that takes advantage of longer primers and a thermostable DNA polymerase isolated fromBacillus caldotenaxYT-G (BcaDNA polymerase). Unlike theE. coliKlenow fragment or modified T7 DNA polymerase, thermostable Bca DNA polymerase allows stable polymerization through templates with high GC content (highly structured regions). The longer 9-mer primers increase the efficiency of incorporation of labeled nucleotides up to 80%. With this kit, random priming is accomplished within 10 minutes to create efficiently-labeled DNA probes. The kit is designed for use with [32P]-α-, [35S]-α-, or [3H]-α-dCTP labels, but it can also be used with biotin, digoxigenin or DNP-labeled dUTPs by replacement of the dNTP mixture. Generally, a probe with specific activityof >109dpm/mg DNA will be obtained with [32P]-α-dCTP (~3,000 Ci/mmol, 111 TBq/mmol).