Takara, 631120, Mir-X™ Inducible miRNA System (Green)

The Mir-X Inducible miRNA System (Green) contains a set of expression vectors that allows you to establish a Tet-regulated microRNA (miRNA) expression system in mammalian cells. An additional feature of the system is that a bright green fluorescent protein marker, ZsGreen1, is coexpressed along with your miRNA, enabling you to verify miRNA expression in intact cells. Inducible expression of the ZsGreen1/pre-miRNA transcript is accomplished by creating a double-stable cell line that expresses the Tet-On Advanced transactivator and that bears your customized pmRi-ZsGreen1 Vector, and then culturing the cell line in the presence of the tetracycline derivative, doxycycline.