Takara, 631152, HEK 293 Tet-Off® Advanced Cell Line
Size: Each, Stable cell lines expressing the tetracycline transactivators Tet-Off and Tet-Off Advanced
Catalog Number: 631152
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| Verified Reactivity | Mouse, Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant fragment of the N-terminus of human MAP2 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | IHC-P - Quality tested WB - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes | This antibody (clone 1D7-B9-C8-F6) works with both Sodium Citrate H.I.E.R. (Cat. No. 928502) and Tris-EDTA pH 9.0 (10mM Tris, 1mM EDTA) antigen retrieval buffers, however, signal to noise ratio is higher using Sodium Citrate. This antibody (clone 1D7-B9-C8-F6) is not suitable for immunocytochemistry. |
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| RRID | AB_3097544 (BioLegend Cat. No. 631151) AB_3097544 (BioLegend Cat. No. 631152) |
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| Structure | MAP2 is a 1,827 amino acid protein with a predicted molecular weight of 199.5 kD. |
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| Distribution | Cellular distribution: plasma membrane, cytoskeleton, nucleus, and cytosol Tissue distribution: Predominantly in the nervous system |
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| Function | Microtubule assembly |
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| Interaction | MAP2 interacts with microtubules. It has also been reported to interact with KNDC1 via KIND2. |
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| Cell Type | Neural Stem Cells |
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| Biology Area | Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells |
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| Molecular Family | Microtubules |
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| Antigen References | De Camilli P, et al. 1984. Neuroscience. 11:817-46. Bloom GS, et al. 1984. J Cell Biol. 98:320-30. Maschietto M, et al. 2013. Front Cell Neurosci. 25:7. La Via L,et al. 2013. Nucelic Acids Res. 41:617-31. Ji B, et al. 2014. Hum Mol Genet. 23:5683-705. Fu X, et al. 2013. J Neurosci. 33:709. |
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| Gene ID | 4133 |
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| UniProt | View information about MAP2 on UniProt.org |
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| Clone | 1D7-B9-C8-F6 |
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| Regulatory Status | RUO |
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| Other Names | Microtubule-associated protein 2 (MAP2) |
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| Isotype | Mouse IgG2b, κ |
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