Description
The Tet-On 3G Inducible Expression System is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. In the EF1α Version, Tet-On 3G transactivator expression is driven by a human elongation factor 1 alpha (EF1α) promoter, which allows expression of the protein in a variety of mammalian cells (such as hematopoietic or stem cells) without the transgene silencing associated with CMV promoters. The system includes a vector set, our highly efficient transfection reagent, Xfect,and Tet System Approved FBS.