Description
Lenti-X Packaging Single Shots (Ecotropic) provide a one-step production method to generate high-titer lentivirus. Each tube of Lenti-X Packaging Single Shots (Ecotropic) contains premeasured, lyophilized XfectTransfection Reagent and an optimized formulation of Lenti-X lentiviral packaging plasmids. High-titer ecotropic lentivirus is produced by adding the lentiviral vector of choice in sterile water to the tube, reconstituting, and then using the mixture to transfect 293T cells (e.g., Lenti-X 293T Cell Line, Cat. # 632180) in a 10-cm dish.