| Verified Reactivity | Human |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human CD102 (ICAM-2) recombinant protein fragment |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | FC - Quality tested ICC, WB - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1 - 10 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes |
|---|
| For western blotting (WB) it is recommended to heat the cell lysate at 37 °C for 30 min rather than boiling it. For immunocytochemistry (ICC), either Fixation Buffer (Cat. No. 420801) or 4% paraformaldehyde followed by methanol permeabilization is recommended. The reactivity of this antibody (clone W22039C) in cell surface flow cytometry is able to be blocked by another BioLegend antibody (clone CBR-IC2/2) (Cat. No. 328502). |
| RRID | AB_3097547 (BioLegend Cat. No. 631351) AB_3097547 (BioLegend Cat. No. 631352) |
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| Structure | 55 kD type I transmembrane glycoprotein, Ig superfamily |
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| Distribution | Vascular endothelial cells, lymphocytes, monocytes, platelets |
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| Function | Adhesion, costimulation |
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| Ligand/Receptor | LFA-1, DC-SIGN, MAC-1 |
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| Cell Type | Endothelial cells, Lymphocytes, Monocytes, Platelets |
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| Biology Area | Immunology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | Weber KSC, et al. 2004. Inflammation. 28:177-88. Barclay AN, et al. 1997. The Leukoycte Antigen Factsbook. Casasnovas JM, et al. 1999. Proc Natl Acad Sci USA. 96:3017-22. Diamonds MS, et al. 1990. J Cell Biol. 111:3129-39. Marchetti L & Engelhardt B. 2020. Vasc Biol. 2:H1-H18. |
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| Gene ID | 3384 |
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| UniProt | View information about CD102 on UniProt.org |
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| Clone | W22039C |
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| Regulatory Status | RUO |
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| Other Names | Intercellular Adhesion Molecule-2, ICAM-2 |
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| Isotype | Mouse IgG2b, κ |
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