| Verified Reactivity | Human, Mouse, Rat |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Recombinant human β-Catenin protein containing residues Serine 33 to Threonine 41. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | WB - Quality tested ICC, IHC-P - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes |
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| This antibody (clone W22009A) cross-reacts with mouse and rat non-phospho β-catenin (active) in western blot (WB). For immunocytochemistry (ICC), 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice-cold methanol or fixation with ice-cold methanol is recommended. For Immunohistochemistry - Paraffin (IHC-P), Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 is recommended for antigen retrieval. |
| RRID | AB_3097567 (BioLegend Cat. No. 631851) AB_3097567 (BioLegend Cat. No. 631852) |
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| Structure | β-catenin is a 781 amino acid protein with a predicted molecular weight of 85 kD. |
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| Distribution | Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon, cortical neurons and breast cancer tissues. |
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| Function | Wnt signaling pathway, regulation of cell-cell adhesion, neurogenesis, transcription, transcription regulation, early embryonic patterning, asymmetric cell division, stem cell renewal, and the epithelial to mesenchymal transition |
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| Interaction | Wnt signaling pathway, apoptotic cleavage of cellular proteins, and EphBEphrinB signaling |
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| Biology Area | Cell Adhesion, Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology |
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| Molecular Family | Adhesion Molecules |
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| Antigen References | Hirano S, et al. 2012. Physiol Rev. 92:597. Yost C, et al. 1996. Genes Dev. 10:1443-54. Morin PJ, et al. 1997. Science. 275:1787-90. |
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| Gene ID | 1499 |
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| UniProt | View information about beta-Catenin on UniProt.org |
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| Clone | W22009A |
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| Regulatory Status | RUO |
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| Other Names | Catenin beta-1, β-catenin, EVR7, CTNNB1, MRD19, NEDSDV, armadillo |
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| Isotype | Rat IgG2b, κ |
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