Takara, 632502, rAcGFP1 Protein

Affinity purified recombinantAequorea coerulescensgreen fluorescent protein (rAcGFP1) can be used as a standard for protein gels, Western blots, and fluorometry. It can also be used as a control for expression studies and for microinjection into cells and tissues. At its N-terminus, rAcGFP1 contains a 6xHN epitope tag, used for purification of the protein by immobilized metal affinity chromatography (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and rAcGFP1, makes it possible to remove the 6xHN polypeptide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties.