Takara, 632507, pRetroQ-DsRed Monomer-N1 Vector

The pRetroQ-DsRed Monomer-N1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a monomeric mutant of theDiscosomasp. red fluorescent protein (DsRed). pRetroQ-DsRed Monomer-N1 allows cloning of genes into the multiple cloning site (MCS) upstream of the DsRed-Monomer coding sequence, which are subsequently expressed as fusions to the N-terminus of the DsRed-Monomer protein. This vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express DsRed-Monomer protein and may be used to produce marker virus to optimize infection protocols.