Takara, 632601, Guide-it™ CRISPR/Cas9 System (Green)

The Guide-it CRISPR/Cas9 System (Green) is a complete system for cloning and expression of a target single guide RNA (sgRNA) for mammalian gene modification studies using CRISPR/Cas9 technology. The pGuide-it-ZsGreen1 Vector is used to simultaneously express Cas9, a target-specific sgRNA, and the exceptionally bright fluorescent protein ZsGreen1. The vector is prelinearized for simple insertion of a target sequence for sgRNA expression from the human U6 promoter; Cas9 nuclease and ZsGreen1 are coexpressed from a bidirectional CMV promoter. This kit also contains all of the components needed for cloning, including ligation mix, necessary controls, and Stellar Competent Cells.