Takara, 632608, AAVpro® CRISPR/Cas9 Helper Free System (AAV2)

The AAVpro CRISPR/Cas9 Helper Free System (AAV2) is a complete system for delivery of genes encoding the components necessary for CRISPR/Cas9-mediated genome editing (i.e., sgRNA and Cas9 nuclease) to mammalian cells. The included AAVpro CRISPR/Cas9 Vector Set consists of two plasmids (pAAV-Guide-it-Up and pAAV-Guide-it-Down) that each contain a truncated, upstream or downstream portion of the Cas9 gene with a 1.6-kb region of homology. Due to the nature of the AAV genome, when target cells are transduced with both viruses (Up and Down), recombination occurs at the region of homology to create a full-length Cas9 gene with an upstream CMV promoter. pAAV-Guide-it-Down is supplied pre-linearized for simple cloning of a target-specific sgRNA sequence under the control of the human U6 promoter. This kit also contains all of the components needed for cloning, including ligation mix, necessary controls, and Stellar Competent Cells, and includes reagents necessary for helper virus-free AAV packaging.