Takara, 632612, pGuide-it-sgRNA1 Vector System

The pGuide-it-sgRNA1 Vector System is a complete system for cloning and expression of a target-specific single guide RNA (sgRNA) for mammalian gene modification studies using CRISPR/Cas9 technology. The pGuide-it-sgRNA1 Vector is used to express a target-specific sgRNA. This vector is pre-linearized for simple insertion of a target sequence for sgRNA expression from the human U6 promoter. It does not contain a selectable marker or fluorescence reporter. pGuide-it-sgRNA1 Vector can be used for transient transfection of mammalian cells. This kit also contains all of the components needed for cloning, including ligation mix, necessary controls, and Stellar Competent Cells.