Takara, 632618, AAVpro® CRISPR/SaCas9 Vector System

The AAVpro CRISPR/SaCas9 Vector System is a complete system for cloning a target single guide RNA (sgRNA) used in CRISPR/Cas9-mediated genome editing in mammalian cells. The pAAV-Guide-it-1 Vector is supplied pre-linearized for simple cloning of a target-specific sgRNA sequence under the control of the human U6 promoter. It also contains the Cas9 gene fromStaphylococcus aureus(SaCas9) expressed from an upstream CMV promoter. SaCas9 has a genome editing efficacy similar to the more commonly usedStreptococcus pyogenesCas9 (SpCas9), but is about 1 kb shorter which allows it to be packaged into a single AAV vector. It is important to note that SaCas9 recognizes a different PAM sequence; please check the user manual for details. This kit also contains all of the components needed for cloning including the ligation mix, necessary controls, and Stellar Competent Cells.