Takara, 632619, AAVpro® CRISPR/SaCas9 Helper Free System (AAV2)

The AAVpro CRISPR/SaCas9 Helper Free System (AAV2) is a complete system for the delivery of genes encoding the components necessary for CRISPR/Cas9-mediated genome editing (i.e., sgRNA and Cas9 nuclease) to mammalian cells. pAAV-Guide-it-1 Vector is supplied prelinearized for simple cloning of a target-specific sgRNA sequence under the control of the human U6 promoter. It also contains the Cas9 gene fromStaphylococcus aureus(SaCas9) expressed from an upstream CMV promoter. SaCas9 can edit the genome with efficiencies similar to those of the more commonly usedStreptococcus pyogenes(SpCas9), but it is about 1 kb shorter, which allows it to be packaged into a single AAV vector. It is important to note that SaCas9 also recognizes a different PAM sequence; please check the user manual for details. This kit also contains all of the components needed for cloning and viral packaging, including ligation mix, necessary controls, Stellar Competent Cells, and reagents necessary for helper virus-free AAV packaging.