Takara, 632633, Lenti-X™ Tet-On® 3G CRISPR/Cas9 System

The Lenti-X Tet-On 3G CRISPR/Cas9 System is designed for Tet-inducible Cas9 expression along with expression of a custom sgRNA. Because Cas9 is only expressed in the presence of doxycycline, genome editing is achieved on demand and without the side effects of persistent Cas9 expression in cell culture. This kit contains all of the components necessary for generating three types of lentivirus: one for constitutive expression of the Tet-On 3G transactivator protein, a second for Tet-inducible Cas9 expression under the PTRE3GV promoter, and a third for constitutive sgRNA expression under the U6 promoter