Takara, 632666, Guide-it™ Long ssDNA Production System v2

The Guide-it Long ssDNA Production System v2 includes all the reagents and materials necessary for on-demand preparation of single-stranded DNA (ssDNA) repair templates of any sequence up to 5,000 nt in length for engineering gene knockins using CRISPR/Cas9 technology or other genome editing tools. It has been demonstrated that ssDNA offers two key advantages over dsDNA templates for precise genome editing applications: greatly reduced toxicity and a much lower likelihood of random or off-target integration. The kit employs a simple and fast method that involves conversion of a dsDNA PCR product into ssDNA via selective digestion of either the sense or the antisense strand. Following digestion, ssDNA products are purified using silica membrane spin columns included with the kit.