Description
Lenti-X SARS-CoV-2 Packaging Single Shots (WT Spike, Truncated) enable streamlined production of high-titer lentivirus pseudotyped with a truncated form of the spike protein from SARS-CoV-2 (Wuhan-Hu-1 isolate) by providing pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of Lenti-X lentiviral packaging plasmids. Pseudovirus production simply involves reconstituting a tube of lyophilized packaging mix with a lentiviral transfer vector of choice in sterile water and adding the mixture to 293T cells (e.g.,Lenti-X 293T Cells, Cat. # 632180) in a 10-cm dish.