Description
Lenti-X SARS-CoV-2 Packaging Mix (WT Spike, Truncated) provides an extremely simple and consistent one-step method for producing high-titer lentivirus pseudotyped with a truncated form of the spike protein from the SARS-CoV-2 virus (Wuhan-Hu-1 isolate). No additional transfection reagent is needed because Lenti-X SARS-CoV-2 Packaging Mix (WT Spike, Truncated) consists of pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of lentiviral packaging plasmids. High-titer virus is produced by simply reconstituting this mixture with your lentiviral transfer vector of choice in sterile water and adding it to 293T cells (e.g.,Lenti-X 293T Cells, Cat. # 632180) in a 10-cm dish.