| Verified Reactivity | Human |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Recombinant fragment of human TFAP2A |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | WB - Quality tested IHC-P, ICC, ICFC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.0625 - 0.5 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.25 - 1.0 µg/mL is suggested. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes |
|---|
| For use of this antibody (clone W20235D) in immunohistochemistry on formalin-fixed paraffin embedded tissue (IHC-P), antigen retrieval with Tris-EDTA pH 9.0 or Citrate Buffer, 10X (Cat. No. 420902) is recommended. For use of this antibody (clone W20235D) in immunocytochemistry (ICC), fixation with either Fixation Buffer (Cat. No. 420801) followed by permeabilization with 0.5% Triton-X or 100% ice-cold methanol is recommended. For use of this antibody (clone W20235D) in intracellular flow cytometry (ICFC), fixation with Fixation Buffer (Cat. No. 420801) followed by True-Phos™ Perm Buffer (Cat. No. 425401) is recommended. This antibody (clone W20235D) is not recommended for mouse or rat reactivity. |
| RRID | AB_3106151 (BioLegend Cat. No. 634351) AB_3106151 (BioLegend Cat. No. 634352) |
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| Structure | Forms homodimer or heterodimer with TFAP2B or TFAP2C to bind to DNA. Contains a PPxY motif which aids interaction with WWOX. |
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| Distribution | Highly expressed in placenta, breast and skin. Located in cell nucleus. |
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| Function | A transcription activator binds to the GC-rich sequence to regulate gene expression. |
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| Interaction | WWOX, EP300, CITED2, and CITED4 |
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| Biology Area | Cell Biology, Cell Proliferation and Viability, Transcription Factors |
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| Molecular Family | Nuclear Markers |
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| Antigen References | Milunsky JM, et al. 2008. Am J Hum Genet. 82:1171-7. Wang WD, et al. 2011. Dev Biol. 360:173-85. Shi D, et al. 2014. Cancer Prev Res. 7:266-77. |
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| Gene ID | 7020 |
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| UniProt | View information about TFAP2A on UniProt.org |
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| Clone | W20235D |
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| Regulatory Status | RUO |
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| Other Names | Transcription factor AP-2-alpha, AP2-alpha, Activator protein 2, AP-2 transcription factor , AP-2, AP-2 alpha, AP2TF, TFAP2, TFAP2alpha, TFAP2 alpha, TFAP |
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| Isotype | Rat IgG2a, κ |
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