Takara, 634359, SMART-Seq® Total RNA Library Prep with ZapR® Depletion

SMART-Seq Total RNA Library Prep with ZapR Depletion generates strand-specific RNA-seq libraries for Illumina sequencing from 250 pg–1 µg inputs of purified total RNA. The protocol for use with this kit was developed to work with either high- or low-quality total RNA, does not require additional rRNA removal methods or kits, and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNA—typically present in high abundance following cDNA synthesis from total RNA inputs—makes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA. Enough material is provided with this kit to perform up to 384 (4 x 96) reactions.