Description
The SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian Components are used with our SMARTer RNA Unique Dual Indexing Kits (Cat. #s 634451, 634452, and 634457) to generate strand-specific RNA-seq libraries for Illumina sequencing from 250 pg—10 ng inputs of purified total RNA. This kit incorporates Takara Bio's proprietary SMART (Switching Mechanism at the 5ˈ end of RNA Template) technology and includes refinements to the SMARTer method for stranded RNA-seq that simplify the library preparation workflow and improve sequencing performance. This method was developed to work with either high- or low-quality total RNA, does not require additional rRNA removal methods or kits, and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNA—typically present in high abundance following cDNA synthesis from total RNA inputs—makes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA. The new library design featured in the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian improves sequencing performance compared to the original SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian, particularly for NextSeq® and MiniSeq™ instruments carrying the two-channel SBS technology.
