Takara, 634451, SMARTer® RNA Unique Dual Index Kit – 24U
Size: 96 Rxns, SMARTer RNA Unique Dual Index Kits enable users to index and multiplex up to 96 NGS libraries
Catalog Number: 634451
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Category
Pack Size96 reactions
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Recombinant full-length human PP2A alpha |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | IHC-P - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes | For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). In human kidney tissue, this antibody is recommended for use at 10 µg/mL, as non-specific staining in tubules may be observed at lower concentrations. This product is not recommended for use in immunocytochemistry (ICC), intracellular flow cytometry (ICFC), or Western blot (WB). |
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| RRID | AB_3683480 (BioLegend Cat. No. 634451) AB_3683480 (BioLegend Cat. No. 634452) |
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| Structure | PP2A alpha is a 309 amino acid protein with a predicted molecular weight of 35.6 kD. Catalytic subunit of protein phosphatase 2A (PP2A). |
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| Distribution | Cytoplasmic and nuclear |
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| Function | Serine/threonine phosphatase which regulates enzymes and signal transduction pathways. |
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| Interaction | PP2A alpha interacts with a wide variety of proteins including AKT, c-Myc, BCL2, cyclin-dependent kinases, MAPK, CD56 and Tau |
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| Cell Type | Neurons |
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| Biology Area | Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Cell Structure, Signal Transduction |
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| Molecular Family | Protein Kinases/Phosphatase |
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| Antigen References | Ronk H, et al. 2022. Cancer Biol Med. 19:1428-39. Reynhout S and Janssens V. 2019. Biochim Biophys Acta Mol Cell Res. 1866:31-50. Lei WL, et al. 2021. Front Cell Dev Biol. 9:638559. Wlodarchak N and Xing Y. 2016. Crit Rev Biochem Mol Biol. 52:162-84. |
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| Gene ID | 5515 |
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| UniProt | View information about PPSA alpha on UniProt.org |
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| Clone | W18203A |
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| Regulatory Status | RUO |
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| Other Names | PPP2CA, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha (PP2AA), Replication protein C (RP-C) |
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| Isotype | Rat IgG2a, κ |
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