Description
The SMARTer RACE 5'/3' Kit allows the synthesis of first-strand cDNA from poly A+ or total RNA via SMART (SwitchingMechanismAt 5' End ofRNATemplate) technology, and facilitates the performance of 5'- and 3'-RACE (Rapid Amplification of cDNA Ends) PCR with the kit's Universal Primer Mix. Our carefully designed, specially-modified SMARTer Oligo preferentially captures the 5' ends of the cDNA during cDNA synthesis. Using this SMARTer Oligo, our procedure enriches cDNA pools for 5' sequences, thus increasing the likelihood you will amplify the entire sequence of your gene.