Takara, 635040, SMART-Seq® mRNA 3’ DE

SMART-Seq mRNA 3′ DE includes the components to generate high-quality cDNA from 1–100 intact cells or ultralow amounts of total RNA. The cDNA generated from each reaction has a cell barcode that allows for pooling downstream. This kit uses the SMART-Seq mRNA technology with locked nucleic acids (LNA) for excellent sensitivity and reproducibility. Additionally, this kit includes a complete set of index primers for downstream library preparation. By focusing the majority of cDNA fragments on the 3′ end of mRNA transcripts, this kit enables differential expression (DE) analysis with fewer reads, reducing the cost of discovery. When combined with the Illumina Nextera® XT DNA Library Preparation Kit, this kit results in a library ready for sequencing on Illumina platforms.