Description
SMART-Seq Total RNA Mid Input includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of poly(A)-purified or ribosomal RNA-depleted RNA. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Indexes are added using a unique dual index kit. The quantity of reagents in this size is sufficient for up to 24 reactions.