Takara, 6601, PCR Mycoplasma Detection Set

The PCR Mycoplasma Detection Set is a primer set designed to detect the presence of mycoplasma in biological materials such as cultured cells. Nested PCR is performed first using primers hybridizing to highly conserved ribosomal genes (16S and 23S rRNA), and then using primers hybridizing to the species-specific spacer region between these ribosomal genes. Verification of species is determined by gel electrophoresis of the amplified products. By performing PCR with this primer set, turnaround time can be shortened from the conventional time of about one week to several hours. This kit allows the highly sensitive and specific detection of the followingMycoplasmaspecies:M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, M. arthritidis, M. neurolyticum, M. hypopneumoniae, M. capricolum and Ureaplasma urealyticum.