Takara, 6653, AAVpro® Helper Free System (AAV5-CRE Recombinase)

This system is used to prepare AAV5 particles that will deliver a Cre recombinase gene cassette to mammalian cells. Cre recombinase is a bacterial protein that recognizes a specific 34-bp sequence (loxPsite) containing an 8-bp core sequence where recombination takes place. The orientation and location of introducedloxPsites allow for Cre-mediated gene inversion, translocation, or deletion.  With this kit, high-titer AAV5 particles can be prepared without a helper virus by simply transfecting HEK 293T cells with the three included plasmids that encode all of the factors necessary to prepare recombinant AAV5 particles.