Description
AAVpro Concentrator provides a simple, highly efficient alternative to ultracentrifugation for concentrating AAV particles of any serotype from cell culture supernatant. In contrast with other concentration methods that involve only precipitation of AAV particles, AAVpro Concentrator incorporates filtration steps to yield AAV preps of higher purity. The scalable mix-and-spin protocol can accommodate a range of input volumes and requires less than one hour of hands-on time. The AAVpro Concentrator reagent is provided as a 4X concentrate, which can be applied in proportion to the amount of culture medium being processed. The kit includes reagents and filtration columns for processing up to 150 ml of culture supernatant for two samples.