Takara, 9115, Agrobacterium tumefaciens LBA4404 Electro-Cells

Agrobacterium tumefaciens (Rhizobium radiobactor)can transfer T-DNA (transfer DNA), which is part of its own Ti plasmid, into host plant cells and insert this DNA into the plant chromosomal DNA. The inserted genes on T-DNA are expressed. By utilizing this gene-transfer mechanism, the binary vector method was invented for plant transformation. In this system, the pathogenic genes of T-DNA in Ti plasmid are replaced with selective marker genes and the exogenous target gene, to transfer the target gene into plant chromosomal DNA by means ofAgrobacterium-mediated gene transfer. This strain ofAgrobacterium tumefaciensincludes the pAL4404 plasmid, which only contains the T-DNA vir region (genes responsible for vir gene induction and T-DNA transfer). LBA4404 is a widely used strain for plant transformation. This product contains competent cells for transformation by electroporation. UsingA. tumefaciensand the binary vector method, you can transform various plants for infection (transfection) experiments.