Takara, RR012A, RNA LA PCR™ Kit (AMV) Ver. 1

The RNA LA PCR Kit is designed to generate longer and more accurate RT-PCR products. The kit uses AMV RT XL as the reverse transcriptase, which allows for efficient synthesis of first-strand cDNA up to 12 kb. AMV RT XL also provides better thermostability than other reverse transcriptases, and retains its activity over a wider range of reaction temperatures. In addition, the kit usesLA Taq,which can synthesize products up to 40 kb in size with 6.5X better fidelity thanTaqPolymerase. Both the reverse transcription and amplification reactions can be performed in a single tube. The components for 3'-RACE are also supplied.