Takara, RR023A, BcaBEST™ RNA PCR Kit Ver. 1.1

TheBcaBEST RNA PCR Kit is designed to perform both reverse transcription and DNA amplification in a single tube. The kit usesBcaBEST DNA Polymerase, which contains both DNA polymerase and reverse transcriptase for first strand cDNA synthesis. In contrast to standard reverse transcriptases,BcaBEST Polymerase has a temperature optimum of 65°C, which permits cDNA synthesis from difficult and highly structured RNA templates.Bca-OptimizedTaqPolymerase is used for second strand synthesis and subsequent PCR. This polymerase utilizes LA PCR technology for improved PCR length and accuracy. Random 9-mers, oligo-dT primers, or a specific downstream primer that acts as an antisense primer in PCR can be used for cDNA synthesis.