Takara, RR057A, PrimeScript™ One Step RT-PCR Kit, Ver.2 (Dye Plus)

The PrimeScript One Step RT-PCR Kit Ver.2 (Dye Plus) is used for one-step RT-PCR in a single tube. There is no need to add reagents during the reaction, minimizing the risk of contamination. Furthermore, electrophoresis loading dyes (blue and yellow) are included in the premix to allow direct analysis by electrophoresis. The bright green color of the reaction mixture allows visual confirmation of gel loading. The kit includes PrimeScript RTase, a reverse transcriptase that provides superior elongation, and TakaraEx TaqHS, a PCR enzyme formulated for hot start PCR that provides high yields. The reaction components are optimized to provide the same level of performance as conventional RT-PCR systems that lack dyes and loading buffer. This kit includes all of the reagents necessary for the synthesis of cDNA from RNA by reverse transcription and amplification of cDNA by PCR.