Takara, RR290, Mouse Feeder Cell Quantification Kit

The Mouse Feeder Cell Quantification Kit is designed to facilitate highly sensitive detection and quantification of genomic DNA derived from residual mouse feeder cells using real-time PCR to analyze genomic DNA extracted from stem cell cultures (e.g., hESC, hiPSC). The kit includes primers for real-time PCR that were designed to detect a mouse mitochondrial (mt) DNA sequence, allowing detection of mouse feeder cells with high sensitivity. The copy number of cellular mtDNA is known to vary depending on cell type, cell line, and differentiation state, and there is a possibility of fluctuation betweenmouse feeder cell lots. The primer set for quantification of mouse mtDNA copy number included in this kit can be used for lot-to-lot correction of mouse feeder cells.