Description
TB GreenPremix Ex Taq(Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB GreenPremixEx Taq(Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR. Cat.#RR42LR and RR42WR are larger package sizes [5 ml and 25 ml (5 x 5 ml), respectively], that contain ROX Reference Dye in the premix.