Takara, T230, Recombinant Cas9 Protein GMP grade

Recombinant Cas9 Protein GMP grade, is a recombinant wild-typeStreptococcus pyogenesCas9 nuclease with a nuclear localization signal (NLS) located at the C-terminus of the protein. It is manufactured and tested in accordance with the following guidelines: "Standards for Manufacturing Control and Quality Control, etc. of Investigational Products (Investigational products GMP)," as notified by the Ministry of Health and Welfare Japan ("PFSB Notification No. 0709002, July 9, 2008"; PFSB = Pharmaceutical and Food Safety Bureau). No animal- or human-derived components are used in the manufacture of this product. The Cas9 protein solution has been verified to be sterile and well-tolerated by mammalian cells when electroporated as a ribonucleoprotein complex (RNP) with a single-guide RNA (sgRNA) for knockout experiments, or as an RNP with a donor repair template for knockin experiments.