Takara, T7103A, Western BLoT Hyper HRP Substrate

The Western BLoT Hyper HRP Substrate is a horseradish peroxidase (HRP) substrate specially developed for Western blotting detection of very low-abundance proteins. Western BLoT Hyper produces a strong, long-lived signal. These features in combination with very low background levels, allows for long exposure times and enables the detection of low-abundance proteins. Additionally, the signal from this product is linear with respect to protein amount over a broad range of concentrations. Western BLoT Hyper HRP is more sensitive than Western BLoT Quant HRP Substrate, and is suitable for detection of low-abundance proteins or in situations when the amount of primary antibody is very limited and a high dilution factor is desired, or when the primary antibody has a relatively low binding affinity. Western BLoT Hyper HRP is also compatible with X-ray film detection, though the limited dynamic range of film will make resulting data less amenable to quantification.