Description
Western BLoT Immuno Booster PF (Protein Free) contains components that enhance antigen-antibody interactions in immunoassays, such as Western blotting, to improve sensitivity and reduce background. This reagent is 100% chemically defined and completely protein-free. The single solution can be used to dilute primary and secondary antibodies and is effective in shortening reaction time, reducing the amount of antibody required, and improving detection of small amounts of antigen. This product does not affect the activity of horseradish peroxidase (HRP) or alkaline phosphatase (AP), and therefore is compatible with enzyme conjugated secondary antibodies and with any detection method (i.e., colorimetric or luminescence). To obtain the highest possible detection signal for Western blotting, use this product in combination with the Western BLoT HRP Substrate Series for HRP chemiluminescence. Because this product is for a protein-free detection system, it is recommended that the Western BLoT Blocking Buffer (Protein Free) (Cat. #T7132A) is used for blocking. Protein-free conditions minimize cross-reactivity that can occur with conventional protein-based blocking buffers, and are therefore ideal for detection of phosphorylated proteins and of low level antigens.
