Thermo Fisher, 21870084, RPMI 1640 Medium, no glutamine, 1000 mL
Quantity: 1000 mL, Unit Size: Each
Catalog Number: 21870084
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Pack Size1000 mL
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| Cell Line | HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas |
|---|---|
| Cell Type | Leukemic Cells |
| Concentration | 1 X |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Product Line | Gibco |
| Product Type | RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) |
| Quantity | 1000 mL |
| Shelf Life | 12 Months From Date of Manufacture |
| Shipping Condition | Room Temperature |
| Classification | Animal Origin-free |
| Form | Liquid |
| Serum Level | Standard Serum Supplementation |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | Phenol Red |
| Without Additives | No Sodium Pyruvate |
| Unit Size | Each |
Answer:No. L-glutamine is omitted from this basal medium.
Answer:1000 mL of liquid RPMI 1640.
Answer:It contains phenol red but does not contain HEPES.
Gibco RPMI 1640 medium without L-glutamine is a 1000 mL liquid basal medium for cultivation of compatible mammalian cell lines and primary cells. RPMI 1640 supplies inorganic salts, glucose, amino acids other than the omitted L-glutamine, and vitamins to support cell metabolism when supplemented for the specific cell-culture protocol. This variant includes phenol red and bicarbonate buffering but omits HEPES and L-glutamine, allowing researchers to select the glutamine supplementation strategy separately. As a basal medium it is not a complete serum-containing formulation; many cell lines also require serum or other growth supplements. Its defined omission of glutamine distinguishes it from ready-to-use RPMI variants that already contain L-glutamine or GlutaMAX.
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