Thermo Fisher, 4482910, Quantifiler™ Trio DNA Quantification Kit

Quantifiler™ Trio DNA Quantification Kit is used in forensic DNA workflows to measure total amplifiable human DNA and human male DNA while also evaluating sample quality in a single real-time PCR assay. The 400-reaction format combines quantitative targets with quality indicators that help laboratories assess degradation and make better decisions about downstream STR analysis. The assay supports selection among autosomal STR, Y-STR, and miniSTR strategies by providing information about both DNA quantity and condition before amplification. TaqMan-based real-time PCR chemistry delivers sensitive and specific measurement, and the workflow is designed to integrate with HID Real-Time PCR Analysis Software for interpretation and decision support. This approach reduces the need to proceed blindly with compromised or low-level samples and helps laboratories allocate extracts to the most appropriate downstream analysis. The kit is particularly suited to human identification and forensic casework laboratories that require quantitative and qualitative information from limited DNA extracts.