Thermo Fisher, 4485715, TaqMan™ RNase P Assay, ABY™ dye/QSY™ probe, primer-limited

Name: TaqMan™ RNase P Assay, ABY™ dye/QSY™ probe, primer-limited
Brand: Thermo Fisher
SKU: 4485715

Quantity: 250 reactions, Unit Size: Each

Catalog Number: 4485715

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Category

Reagent Bottles

Pack Size250 reactions

TagsPCR Equipment and Supplies PCR Supplies Real Time PCR Products Real Time PCR Assays and Arrays

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 250 reactions
Unit Size: Each
• The Applied Biosystems™ TaqMan™ RNase P Assay with ABY™ dye-labeled QSY™ probe (primer limited) provides a pre-formulated assay for quantitating human RNase P.
• This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. It consists of an ABY™ dye-labeled QSY™ probe plus sequence-specific forward and reverse primers.
• The assay can be used for multiplex or singleplex PCR reactions, and it is primer limited, so it can be used with samples that overexpress RNase P.
• Eliminate assay design time using pre-designed primers and pre-designed ABY™ dye-labeled TaqMan™ probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ or VIC™ dye-labeled predesigned gene expression assays
• Formulated at a primer-limited concentration for use in multiplex reactions
• Save time in assay design, formulation, and testing by using the TaqMan™ RNase P Assay as your control when quantifying gene expression.
• This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.
• In order to facilitate multiplex experiments, the assay formulation is primer limited, allowing the assay to be used with high RNase P expressors. The ABY™ dye is optimized for use with the 3rd filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments.

Specifications

General

Accession Number	NR_002312.1
Amplicon Size	87
Format	Tube
Gene Symbol	RNase P (RPPH1)
Internal Probe Modification	QSY™ Non-Fluorescent Quencher (3')
No. of Reactions	250 reactions
Product Line	TaqMan
Product Type	Real Time PCR Reagents
Quantity	250 reactions
Species	Human
Target	Ribonuclease P RNA component H1
Detection Method	Primer-probe
For Use With (Application)	Real Time PCR (qPCR)
GC-Rich PCR Performance	High
Label or Dye	ABY
PCR Method	qPCR
Unit Size	Each

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