Thermo Fisher, A26343, QuantStudio™ 3/5 10-Dye Spectral Calibration Kit, 96-well, 0.2 mL

QuantStudio™ 3/5 10-Dye Spectral Calibration Kit, 96-well, 0.2 mL is designed for spectral calibration of QuantStudio 3 and QuantStudio 5 real-time PCR systems equipped with a 96-well 0.2 mL block. The kit establishes pure dye spectra and multicomponent values so the instrument can distinguish fluorescence from ten supported dyes when multiple reporters are excited and detected within the same optical system. The kit covers FAM, VIC, ROX, SYBR, ABY, JUN, MUSTANG PURPLE, TAMRA, NED and Cy5 dyes. It contains three preloaded 96-well 0.2 mL spectral calibration plates: Plate 1 contains FAM, VIC, ROX and SYBR dyes; Plate 2 contains ABY, JUN and MUSTANG PURPLE; and Plate 3 contains TAMRA, NED and Cy5. Because the dyes are supplied in preloaded plates, calibration can be performed without preparing individual dye solutions or manually dispensing standards. Store the kit at −20 °C ±5 °C and protect the calibration materials as directed. This calibration is used when installing, maintaining or verifying the optical system so subsequent multiplex qPCR data can be accurately separated into the fluorescence contribution from each dye. The three-plate structure covers the full ten-dye optical set used by the 0.2 mL QuantStudio 3/5 configuration. Running the complete kit gives the instrument software reference spectra for correcting spectral overlap before experimental multiplex assays are analyzed.