Description
Quantity: 1 mg
Unit Size: Each
Alexa Fluor™ 488 Cadaverine is useful as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety. Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 488 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Alexa Fluor™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection.Detailed information about this AlexaFluor™ cadaverine:• Fluorophore label: Alexa Fluor™ 488 dye• Reactive group: cadaverine• Reactivity: carboxylic acids, aldehydes, and ketones (and glutamine residues through an enzyme-catalyzed transamidation reaction)• Ex/Em of the conjugate: 493/516 nm• Extinction coefficient: 73,000 M^-1cm^-1• Spectrally similar dyes: FITC, GFP• Molecular weight: 640.61 g/molCell Tracking and Tracing ApplicationsAlexa Fluor™ cadaverines make excellent fluorescent polar tracers because they are bright, small, and water soluble. Since they contain an aldehyde-fixable functional group, they can be fixed in cells by treatment with formaldehyde or glutaraldehyde. They are easily loaded into cells by microinjection, infusion from patch pipette, or uptake induced by our Influx™ Pinocytic Cell-Loading Reagent (Cat. No. I14402).Protein Labeling ApplicationsAlexa Fluor™ cadaverines can be used as reactive molecules for adding a fluorescent label to carboxylic acids using a coupling agent such as a carbodiimide; they do not spontaneously react with carboxylic acids in solution. They do, however, react spontaneously with the common amine-reactive functional groups, including succinimidyl esters and isothiocyanates. The amine-containing Alexa Fluor™ cadaverines can also be used to label glutamine residues in some proteins and peptides via an enzyme-catalyzed transamidation reaction.Learn More About Protein and Antibody LabelingWe offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See our Antibody Labeling kits or use our Labeling Chemistry Selection Tool for other choices. To learn more about our labeling kits, read Kits for Labeling Proteins and Nucleic Acids–Section 1.2 in The Molecular Probes™ Handbook.We’ll Make a Custom Conjugate for YouIf you can’t find what you’re looking for in our online catalog, we’ll prepare a custom antibody or protein conjugate for you. Our ustom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.Dissolve in water to prepare a stock concentrations of 1 to 10 mg/mL.Related ProductsDMSO (dimethylsulfoxide) (Cat. No. D12345)Antibody Conjugate Purification Kit for 0.5–1 mg (Cat. No. A33086)Antibody Conjugate Purification Kit for 20–50 μg (Cat. No. A33087)Antibody Conjugate Purification kit for 50–100 μg (Cat. No. A33088)
