Thermo Fisher, D23806, Dihydrorhodamine 123
Quantity: 1 mL, Unit Size: Each
Catalog Number: D23806
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Pack Size1 mL
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| Quantity | 1 mL |
|---|---|
| Unit Size | Each |
Answer:It forms fluorescent rhodamine 123, generating a green signal associated with mitochondria.
Answer:The package contains 1 mL.
Answer:No. Its fluorescence reflects oxidation-dependent conversion and should be interpreted with appropriate controls.
Dihydrorhodamine 123 is designed as a cell-permeant indicator for investigating reactive oxygen species through oxidation-dependent fluorescence. The uncharged reduced dye is initially nonfluorescent and can passively cross cellular membranes. After oxidation, it forms cationic rhodamine 123, a green fluorescent product that accumulates in mitochondria. This conversion enables experiments that monitor changes in cellular oxidative conditions by fluorescence microscopy or other compatible fluorescence detection approaches. Because the readout is generated by chemical oxidation, measurements require appropriate experimental controls to distinguish treatment-related changes from photooxidation and other handling effects. The supplied configuration contains 1 mL of reagent, not a dry 1 mg or 5 mg powder pack. The fluorescent oxidation product has an excitation maximum near 507 nm and an emission maximum near 529 nm, providing a green-channel readout. Dihydrorhodamine 123 is an indicator of oxidation-dependent signal formation rather than a uniquely selective probe for one individual ROS species, so results should be interpreted in the context of experimental conditions and controls.
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