Thermo Fisher, EP0402, Taq DNA Polymerase, recombinant (5 U/μL)

Name: Taq DNA Polymerase, recombinant (5 U/μL)
Brand: Thermo Fisher
SKU: EP0402

Quantity: 500 Units, Unit Size: Each, Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5' to 3' synthesis of DNA, has no detectable 3' to 5' exonuclease (proofreading) activity, and possesses low 5' to 3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Rec

Catalog Number: EP0402

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Category

DNA Polymerases

Pack Size500 units

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products Standard PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 Units
Unit Size: Each
Features
• Thermostable—half life is more than 40 min at 95°C
• Generates PCR products with 3'-dA overhangs
• Supplied with two buffers: 10XTaqBuffer with KCl and 10XTaqBuffer with (NH_4)_2SO_4
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescent-labeled nucleotides)Applications
• Routine PCR amplification of DNA fragments up to 5 kb
• High throughput PCR
• DNA labelingNotes
• The error rate ofTaqDNA Polymerase in PCR is 2.2 x 10^-5errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 10^4. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
• 10XTaqBuffer without Detergent is recommended for microarray experiments.
• 10XTaqBuffer with (NH_4)_2SO_4allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.

Specifications

General

Fidelity (vs. Taq)	1X
Hot Start	No
Overhang	3'-A
Polymerase	<i>Taq</i> DNA Polymerase
Product Type	<i>Taq</i> DNA Polymerase (Recombinant)
Quantity	500 Units
Reaction Format	Separate Components
Shipping Condition	Dry Ice
Concentration	5 U/μL
For Use With (Application)	Standard PCR
GC-Rich PCR Performance	Low
Reaction Speed	Standard
Unit Size	Each

FAQs

Question: How much enzyme is supplied?

Answer:This configuration contains 500 units at 5 U/µL.

Question: Does this Taq polymerase have proofreading activity?

Answer:No. It has no detectable 3′→5′ proofreading exonuclease activity.

Question: What type of PCR is it intended for?

Answer:It is intended for routine DNA amplification where standard Taq performance is appropriate.

Application Note

Recombinant Taq DNA Polymerase is a thermostable enzyme for routine PCR amplification of DNA templates. This configuration provides 500 units at 5 U/µL. The enzyme catalyzes 5′→3′ DNA synthesis and has no detectable 3′→5′ proofreading exonuclease activity, so it is appropriate for standard amplification workflows where high proofreading fidelity is not required. Taq also has low 5′→3′ exonuclease activity and commonly adds a single extra adenine to the 3′ ends of PCR products, which can be useful for TA cloning. The enzyme is supplied with reaction buffer for conventional PCR setup and is suitable for amplification from genomic, plasmid, and cDNA templates when primers and cycling conditions are optimized for the target. Use an appropriate magnesium concentration and extension time based on amplicon length, template complexity, and downstream application.

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