Thermo Fisher, EP0441, RevertAid Reverse Transcriptase (200 U/μL), 10,000 Units

Quantity: 10,000 Units
Unit Size: Each
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids that is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.Features of RevertAid Reverse Transcriptase include:• Efficient synthesis of full-length first strand cDNA up to 13 kb• Optimum activity at 42°C• Active up to 50°C• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)Applications• First strand cDNA synthesis for RT-PCR and real-time RT-PCR• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extensionSourceRevertAid Reverse Transcriptase is sourced from E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.