| Fidelity (vs. Taq) | 1X |
|---|---|
| Hot Start | No |
| No. of Reactions | 200 Reactions |
| Overhang | 3'-A |
| Polymerase | <i>Taq</i> DNA Polymerase |
| Product Type | PCR Master Mix |
| Quantity | 200 reactions |
| Reaction Format | SuperMix or Master Mix |
| Starting Material | DNA |
| Concentration | 2X |
| For Use With (Application) | Standard PCR |
| GC-Rich PCR Performance | Low |
| Reaction Speed | Standard |
| Unit Size | Each |
Answer:It contains Taq DNA polymerase, dNTPs, buffer components, and the core reagents required for PCR.
Answer:Users add DNA template, primers, and water as required by the assay.
Answer:This configuration provides material for approximately 200 PCR reactions.
PCR Master Mix (2X) is used for routine endpoint PCR amplification with a simplified reaction setup. The ready-to-use 2X formulation contains Taq DNA polymerase, dNTPs, buffer components, and the other core reagents needed for PCR, leaving the user to add primers, DNA template, and water to the final reaction. Using a premixed formulation reduces pipetting steps, lowers the chance of component omission, and improves consistency across replicate reactions. The kit supports standard amplification of DNA fragments for cloning, genotyping, screening, and other molecular biology applications. This configuration provides reagents for approximately 200 reactions and includes four 1.25 mL tubes of 2X PCR Master Mix together with nuclease-free water. The master mix is stored frozen at approximately –20°C and can be aliquoted if repeated access is expected.
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