Thermo Fisher, K1232, CloneJET PCR Cloning Kit, 40 Reactions

Name: CloneJET PCR Cloning Kit, 40 Reactions
Brand: Thermo Fisher
SKU: K1232

No. of Reactions: 40 Reactions, Unit Size: Each, CloneJET PCR Cloning Kit is an advanced positive selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any other blunt or sticky-end DNA fragment can be cloned. It is ideal for phosphorylated or non-phosphorylated DNA fragments. Ligation into the included positive selection vector takes only 5 minutes, yielding more than 99% recombinant clones. Blunt-ended PCR products generated with a proofre

Catalog Number: K1232

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Category

DNA Polymerases

Pack Size40 reactions

TagsBiochemical Reagents DNA Reagents PCR Cloning Kits

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

No. of Reactions: 40 Reactions
Unit Size: Each
HighlightsThe CloneJET PCR Cloning Kit contains a novel, ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularized pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host E. coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.For convenience in mapping and manipulation of the insert, the pJET1.2/blunt cloning vector multiple cloning site contains two BglII recognition sequences that flank the insertion site. In addition, the vector contains a T7 promoter for in vitro and in vivo transcription as well as sequencing of the insert.Highlights
• Fast- PCR cloning in only 5 minutes
• Highest efficiency- > 99% of positive clones
• No cloning background- positive selection vector
• Versatile- ideal for blunt-end or sticky-end cloning
• Economical- no expensive blue/white screening

Specifications

General

Antibiotic Resistance Bacterial	Ampicillin (AmpR)
Cloning Method	Restriction Enzyme⁄ MCS, Blunt PCR
For Use With (Application)	PCR Cloning
No. of Reactions	40 Reactions
Product Line	CloneJET
Product Type	Cloning Kit
Quantity	40 Reactions
Sample Type	PCR Products
Vector	pJET1.2/blunt
Format	Kit
Unit Size	Each

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