Thermo Fisher, K250020, pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 <i>E. coli</i>

Quantity: 20 Reactions
Unit Size: Each
• Contains minimal MCS, so sequence more of insert and less of vector
• Patentedattsites, pCR™8 vector to create entry clone, which facilitates easy access via Gateway recombination cloning to variety of expression vectors without tedious sub-cloning, saving valuable research time
• attsites for rapid recombination into a variety of Gateway destination vectors
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site
• Spectinomycin resistance gene for robust selection inE. coli
• EcoRI sites flanking the PCR product insertion site for easy excision of insertsCloning,Gateway Cloning,PCR Cloning